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Brucella melitensis 1

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Nanoconjugate vaccine 1

Protein glycan coupling technology (PGCT) 1

attenuation 1

cellular fatty acids 1

dendrogram 1

differential diagnosis 1

gas chromatography 1

live attenuated vaccine 1

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Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine against Brucellamelitensis Article

Jing Huang, Yufei Wang, Kangfeng Wang, Shulei Li, Peng Sun, Yan Guo, Jiankai Liu, Ruifu Yang, Ming Zeng, Chao Pan, Hengliang Wang, Li Zhu

Engineering 2023, Volume 29, Issue 10,   Pages 95-109 doi: 10.1016/j.eng.2023.04.007

Abstract:

Brucellosis, caused by Brucella, is one of the most common zoonosis.dual-antigen nanoconjugate vaccine containing both polysaccharide and protein antigens against Brucellaresponse against the two antigens and remarkable protective effect in nonlethal
and lethal Brucellananoconjugate vaccine enhanced both T helper 1 cell (Th1) and Th2 immune responses and protected mice from BrucellaTo our knowledge, this is the first Brucella
vaccine bearing diverse antigens, including

Keywords： Protein glycan coupling technology (PGCT)     Dual-antigen     Nanoconjugate vaccine     Brucella melitensis    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAO MS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 216-219 doi: 10.1007/s11684-010-0037-2

Abstract: To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAsinformation in typing Brucella, 19 reference strains were subjected to CFAs study.After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylatedTyping Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the

Keywords： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Attenuation mechanism of

Yuanqiang ZHENG,Yanchun SHI,Chang AN,Ruisheng LI,Dongjun LIU,Yuehua KE,Kairong MAO,Mingjuan YANG,Zeliang CHEN,Shorgan BOU

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 331-340 doi: 10.15302/J-FASE-2014032

Abstract: Brucellosis is a worldwide zoonosis. Vaccination is the most efficient means to prevent and control brucellosis. The current licensed attenuated vaccines for animal use were developed by sequential passage in non-natural hosts that decreased virulence in its original hosts. The attenuation mechanism of these strains remains largely unknown. In the present study, we sequenced the genome of vaccine strain M5-10. Sequence analysis showed that a large number of genetic changes occurred in the vaccine strains. A total of 2854 genetic polymorphic sites, including 2548 SNP, 241 INDEL and 65 MNV were identified. Of the 2074 SNPs in coding regions, 1310 (63.2%) were non-synonymous SNPs. Gene number, percent and N/S ratios were disproportionally distributed among the cog categories. Genetic polymorphic sites were identified in genes of the operon, flagella synthesis, and virulence regulating systems. These data indicate that changes in some cog categories and virulence genes might result in the attenuation. These attenuation mechanisms also have implications for screening and development of new vaccine strains. The genetic changes in the genome represent candidate sites for differential diagnosis between these vaccine strains and other virulence ones. Transcription analysis of virulence genes showed that expression of , were reduced in M5-10 strain when compared with that in 16M. A duplex PCR targeting and was successfully used to differentiate between M5-10 and the virulent 16M strain. The genome re-sequencing technique represents a strong strategy not only for evaluation of vaccines, but also for development of new vaccines.

Keywords： Brucella     live attenuated vaccine     attenuation     differential diagnosis    

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Title Author Date Type Operation

Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine against Brucellamelitensis

Jing Huang, Yufei Wang, Kangfeng Wang, Shulei Li, Peng Sun, Yan Guo, Jiankai Liu, Ruifu Yang, Ming Zeng, Chao Pan, Hengliang Wang, Li Zhu

Journal Article

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAO MS,

Journal Article

Attenuation mechanism of

Yuanqiang ZHENG,Yanchun SHI,Chang AN,Ruisheng LI,Dongjun LIU,Yuehua KE,Kairong MAO,Mingjuan YANG,Zeliang CHEN,Shorgan BOU

Journal Article